P21(WAF1) inhibits the growth of leukemia cell line K562 and decreases its sensitivity to Vp16 / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 19-22, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-261448
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of p21(WAF1) on the proliferation and the sensitivity to Vp16 of leukemia cell line K562.</p><p><b>METHODS</b>A p21(WAF1) retroviral expression vector pLXSN-p21(WAF1) was constructed by FuGENE 6, pLXSN-p21(WAF1) and pLXSN-neo, and transfected into p21(WAF1) defect leukemia cell line K562. After selected with G418, K562-p21(WAF1) cell clones that stably expressed p21(WAF1) were isolated. The ectopic expressions of p21(WAF1) mRNA and protein in K562-p21(WAF1) were identified by RT-PCR and Western b1ot. The cell growth rate was tested by trypan blue dye, the cell cycle by FCM and the sensitivity to Vpl6 by cell count and MTT assay.</p><p><b>RESULTS</b>The expression of p21(WAF1) protein and mRNA could be detected in K562-p21(WAF1) cells. A strong inhibition of cell proliferation was observed in K562-p21(WAF1) cell as compared with that of the control. The cell number in G(0)/G(1) phase was remarkably increased. The sensitivity to Vpl6 decreased, the IC (50) of K562-neo cells was (56.4 +/- 6.5) microgram/ml, and that of K562-p21(WAF1) cells was (131.0 +/- 8.7) microgram/ml (P < 0.01).</p><p><b>CONCLUSION</b>p21(WAF1) can inhibit the proliferation of leukemia cell and decrease its sensitivity to Vp16.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Time Factors
/
RNA, Messenger
/
Gene Expression
/
Cell Division
/
Cell Survival
/
Blotting, Western
/
Resting Phase, Cell Cycle
/
G1 Phase
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2002
Type:
Article
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