Stimulation of adenosine A1 receptor attenuates angiotensin II induced myocardial hypertrophy in neonatal rats via the extracellular signal-regulated kinase signal pathways / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 698-703, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-261504
ABSTRACT
<p><b>OBJECTIVE</b>To observe the impact of adenosine A1 receptor stimulation on extracellular signal-regulated kinase 1/2 (ERK1/2) signal pathways on angiotensin II (AngII) stimulated cardiomyocytes of neonatal rats in vitro.</p><p><b>METHODS</b>Cardiomyocytes of neonatal rats were cultured in vitro. Cardiomyocytes hypertrophy was induced by AngII (0.1 µmol/L). The antihypertrophic effect of adenosine A1 receptor stimulation via adenosine A1 receptor agonist R-PIA (1 µmol/L) was observed in the presence or absence of ERK1/2 inhibitor 1, 4-Diamino-2, 3-dicyano-1, 4-bis(o-aminophenylmercapto) butadiene (U0126) 1 µmol/L, PKC inhibitor Ro-31-8220 (50 nmol/L), and pertussis toxin (PTX, 5 mg/L). The total protein content was assayed by the method of Lowry. The expression of mRNA of atrial natriuretic peptide (ANP) was determined by RT-PCR. [Ca(2+)]i was measured by confocal microscope using Fluo-3/AM as fluorescent indicator. The relative expression of ERK1/2 was determined by Western blot.</p><p><b>RESULTS</b>Compared with normal control group, AngII induced significant cardiomyocyte hypertrophy. Compared with AngII group, R-PIA significantly inhibited AngII-induced increase of the protein content, cardiomyocytes volume and expression of ERK1/2, calcium ion fluorescence intensity, similar as U0126 and Ro-31-8220. The inhibitory effects on AngII induced cardiomyocytes hypertrophy of R-PIA were lost when preincubated with PTX.</p><p><b>CONCLUSION</b>Adenosine A1 receptor can inhibit AngII induced cardiomyocyte hypertrophy through downregulating ERK signal pathways and reducing intracellular Ca(2+).</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Angiotensin II
/
Cells, Cultured
/
Calcium
/
Rats, Sprague-Dawley
/
Cardiomegaly
/
MAP Kinase Signaling System
/
Myocytes, Cardiac
/
Receptor, Adenosine A1
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2013
Type:
Article
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