Study on the relationship between the polymorphisms and secondary structure of tat exon-1 gene and HIV/ AIDS progress in subtype B' and B'/C / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 968-972, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-261696
ABSTRACT
<p><b>OBJECTIVE</b>To study the polymorphisms and secondary structure of human immunodeficiency virus (HIV-1) tat exon 1 among subtype B' and B'/C HIV-1 infected people in China and to explore the relationship between the polymorphism of tat exon 1 and the disease progression.</p><p><b>METHODS</b>8 subtype B' and 5 B'/C HIV-1 infected patients with slow disease progression were selected from Liaoning, Jilin and Yunnan province. 26 subtype B' and 9 B'/C HIV-1 infected patients with similar sex, age but with typical disease progression were selected. Provirus was extracted from the whole blood. The gene sequences of the Tat exon 1 were amplified by nest-polymerase chain reaction (nest-PCR). Products were purified and sequenced directly. The sequences were aligned, translated, amino acid substitution were analyzed and secondary structures were predicted.</p><p><b>RESULTS</b>Many amino acid substitution could be found in the exon 1 of Tat in HIV-1 subtype B' and B'/C recombinant strain infected persons with different disease progression except A58T,none of them showed definitely relationship with HIV viral load and disease progression. 23N, 31S, 32Y and 46F were subtype-specific substitutions. No characteristic secondary structure of exon 1 of Tat was found.</p><p><b>CONCLUSION</b>Some of the mutations of tat exon 1 might be related to HIV viral load and disease progression. However, there was no relationship found between the secondary structure of Tat protein and the disease progression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Polymorphism, Genetic
/
Genes, tat
/
HIV Infections
/
Exons
/
Acquired Immunodeficiency Syndrome
/
Disease Progression
/
Viral Load
/
Amino Acid Substitution
/
Human Immunodeficiency Virus Proteins
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Epidemiology
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS