Detection of TERC gene amplification by fluorescence in-situ hybridization in cervical intraepithelial lesions / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 182-186, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-261832
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility and practical value of fluorescence in situ hybridization (FISH) detection of TERC gene amplification in cervical intraepithelial lesions (CIN) and squamous cell carcinoma (SCC).</p><p><b>METHODS</b>Tissue microarray was constructed to cover 150 cases of various cervical conditions, including 24 cases of normal cervical mucosa, 78 cases of CINs (CINI, 25 cases; CINII, 21 cases and CINIII, 32 cases) and 48 cases of SCC. FISH was used to detect TERC gene amplification.</p><p><b>RESULTS</b>TERC gene amplification was detected in 8% (2/25) CINI, 47.6% (10/21) CINII, 71.9% (23/32) CINIII and 87.5% (42/48) SCC. There were significant differences among these groups (P < 0.05). The amplification rates of TERC gene in SCC, CINIII and CINII were significantly higher than those of normal cervical epithelium and CINI (P < 0.05). Significant differences were also observed among CINI and CINII, CINIII and SCC (P < 0.05), and between CINII and SCC (P < 0.05). There were no significant differences between normal cervical epithelium and CINI, CINII and CIN III, and between CINIII and SCC (P > 0.05). FISH detection of amplification of TERC gene in CINI and CINII-III demonstrated the following statistics sensitivity of 62.3%, specificity of 92.0%, accuracy of 71.8%, positive and negative predictive values of 94.3% and 53.5%, respectively.</p><p><b>CONCLUSIONS</b>FISH detection is a reliable method in detecting TERC gene amplification using paraffin tissue sections. When histological evaluation becomes difficult, TERC amplification detectable by FISH may offer a diagnostic distinction of CINI from CINII. Moreover, TERC amplification may be used as a biomarker in predicting CIN progression to invasive cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA
/
Carcinoma, Squamous Cell
/
Biomarkers, Tumor
/
Adenoma
/
Uterine Cervical Dysplasia
/
Uterine Cervical Neoplasms
/
Gene Amplification
/
Sensitivity and Specificity
/
In Situ Hybridization, Fluorescence
/
Disease Progression
Type of study:
Diagnostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2011
Type:
Article
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