Determination of ilexside II in leaves of Ilex cornuta by HPLC-ELSD / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a HPLC-ELSD method for determination of ilexside II in leaves of Ilex cornuta.</p><p><b>METHOD</b>The separation was performed on a Waters Syemmetry Shield RP18 column (4.6 mm x 250 mm, 5 microm) and methanol-water was used as mobile phase in gradient elution. The flow was 1.0 mL x min(-1), and the temperature was set at 35 degrees C.</p><p><b>RESULT</b>The calibration curve showed good linearity in the test range (R2 = 0.9997). And the mean recovery was 101.3%, RSD was 2.0% (n=6).</p><p><b>CONCLUSION</b>The developed HPLC-ELSD method was accurate and reproducible, and can be used for the determination of ilexside II in leaves of Ilex cornuta.</p>