Influence of astragalosides and Panax notoginseng saponins compatibility on MMP-9 and TIMP-1 after cerebral ischemia-reperfusion in mice / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2187-2191, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-262195
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of astragalosides (AST) and Panax notoginseng saponins (PNS) compatibility on the expression of matrix metalloproteinases-9 (MMP-9) and tissue inhibitor of metal11oproteinase-1 (TIMP-1) after cerebral ischemia/reperfusion (I/R) injury in mice, to probe into its anti-ischemic brain injury protection mechanism.</p><p><b>METHOD</b>C57BL/6N mice were randomly divided into sham-operation group, model group, AST and PNS compatibility of high, medium and low-dose group, AST group, PNS group and edaravone group. Cerebral ischemia-reperfusion injury were prepared by bilateral common carotid artery ligation for 20 min followed by 24 hours reperfusion after administration for 4 days. Pathomorphism was detected with HE staining and the expression of MMP-9 and TIMP-1 protein in brain was detected by western-blot.</p><p><b>RESULT</b>The neuronal survival rate in the drug groups was significantly higher than the control group (P < 0.01), and the effect of the-middle dose compatibility group was more obvious. Factorial analysis manifested that AST110 mg x kg(-1) and PNS115 mg x kg(-1) compatibility had a synergistic interaction (P < 0.01). The expression of MMP-9 protein in the drug groups was lower than the model group significantly (P < 0.01 or P < 0.05), but the expression of TIMP-1 protein was higher than the model group significantly (P < 0.01 or P < 0.05), and the effect of the-middle dose compatibility group was more obvious, the two drugs had the stacking interaction (P < 0.05).</p><p><b>CONCLUSION</b>AST110 mg x kg(-1) and PNS115 mg x kg(-1) compatibility has a synergistic effect against ischemia-reperfusion injury in mice by accommodating MMP-9/TIMP-1 probably.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Drugs, Chinese Herbal
/
Reperfusion Injury
/
Brain Ischemia
/
Chemistry
/
Blotting, Western
/
Tissue Inhibitor of Metalloproteinase-1
/
Matrix Metalloproteinase 9
/
Astragalus Plant
/
Therapeutic Uses
/
Drug Therapy
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Type:
Article
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