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Research on antiproliferative effect of flavones isolated from Laggera pterodonta / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2171-2174, 2010.
Article in Chinese | WPRIM | ID: wpr-262199
ABSTRACT
<p><b>OBJECTIVE</b>To research the cytotoxicity and in vitro antiproliferative effect of the six flavone compounds extracted from Laggera pterodonta.</p><p><b>METHOD</b>The cytotoxicity on the normal cells and antiproliferative effect on tumor cells were tested by MTT assay, and then the preliminary structure-activity relationship was analysed. The phase distribution of the cell cycle and apoptosis rate were analyzed by flow cytometry.</p><p><b>RESULT</b>The results of MTT assay showed 5,7,3',4'-tetramethoxy-3-hydroxyflavone and chrysosplenetin B inhibited growth of A549 and Hela cells significantly with a dose dependent mode, while exhibited low cytotoxicity to the two normal cells, Vero and EVC304. Both compounds contain ortho-phenolic methoxyl moietys in their structures. Flow cytometry analysis revealed that Hela cells treated with increasing quantities of chrysosplenetin B increased the percentage of cells in the G2/M phase, and Hela and A549 cells treated with increasing quantites of the 5,7,3',4'-tetramethoxy-3-hydroxyflavone and chrysosplenetin B increased the apoptosis rates.</p><p><b>CONCLUSION</b>The 5,7,3',4'-tetramethoxy-3-hydroxyflavone and chrysosplenetin B extracted from L. pterodonta showed high antiproliferative effect on cancer cells with low cytotoxicity on normal cells, and took the effects on A549 and Hela cells through the hold-up of the G2/M phase of the cell cycle and induction of the apoptosis.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Flavonoids / Vero Cells / HeLa Cells / Cell Line / Chlorocebus aethiops / Chemistry / Asteraceae / Cell Line, Tumor / Flavones Limits: Animals / Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Flavonoids / Vero Cells / HeLa Cells / Cell Line / Chlorocebus aethiops / Chemistry / Asteraceae / Cell Line, Tumor / Flavones Limits: Animals / Humans Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2010 Type: Article