Assay of andrographolide bulk drug by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2113-2115, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-262212
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for the determination of andrographolide bulk drug by HPLC.</p><p><b>METHOD</b>The condition of HPLC was Diamonsil C18 (2) column (4.6 mm x 250 mm, 5 microm) with methanol-water (6040) as a mobile phase. The flow rate was 1.0 mL x min(-1) and the detection wavelength was 235 nm.</p><p><b>RESULT</b>The standard curve was linear in the range of 0.5-5.0 microg and the correlation coefficient was 1.000. The average recoveries of the low, middle and high concentrations were 99.1%, 99.5% and 100.8%, respectively.</p><p><b>CONCLUSION</b>The method is simple, precise and accurate, and it can be used for the quality control of andrographolide bulk drug.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Drugs, Chinese Herbal
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Diterpenes
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Type:
Article
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