Quantitative determination of forsythiaside in Forsythia suspensa / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a quantitative determination of Forsythia suspensa and to provide scientific basis for its quality control.</p><p><b>METHOD</b>By HPLC, the separation was performed on a Zorebax Eclipse XDB-C18 column (4.6 mm x 250 mm, 5 microm) at 25 degrees C using a isocratic elution of acetonitrile-water (containing 0.4% glacial acetic acid, 1585). The detection wavelength was 330 nm.</p><p><b>RESULT</b>The calibration curve showed a good linearity (r = 0.9998) within test range of 0.202-1.515 microg. And the average recovery was 98.54% with RSD 1.1%. The content range of forsythoside A in 11 batches of Forsythiae Fructus was 0.200%-1.681%.</p><p><b>CONCLUSION</b>The developed method is simple, accurate, specific, and with good repeatability. It is suitable for quality evaluation of Forsythiae Fructus.</p>