Simultaneous determination of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2061-2064, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-262226
ABSTRACT
<p><b>OBJECTIVE</b>To develop a HPLC method to determine the content of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex simultaneously. MEHTOD The separations were carried out at 25 degrees C on a Phenomenex Gemini C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitril and water containing 0.1% phosphoric acid in gradient mode. The flow rate was 1.0 mL x min(-1), detection wavelengthes were 345 nm for jatrorrhizine , palmatine, berberine and 210 nm for obacunone.</p><p><b>RESULT</b>The average recoveries of jatrorrhizine, palmatine, berberine, and obacunone were 98.94%, 101.17%, 96.22% and 98.90%, respectively.</p><p><b>CONCLUSION</b>The method is simple, accurate and repeatable, and can be used in content determination of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Benzoxepins
/
Berberine
/
Berberine Alkaloids
/
Drugs, Chinese Herbal
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Phellodendron
/
Limonins
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Type:
Article
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