Your browser doesn't support javascript.
loading
Simultaneous determination of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2061-2064, 2010.
Article in Chinese | WPRIM | ID: wpr-262226
ABSTRACT
<p><b>OBJECTIVE</b>To develop a HPLC method to determine the content of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex simultaneously. MEHTOD The separations were carried out at 25 degrees C on a Phenomenex Gemini C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitril and water containing 0.1% phosphoric acid in gradient mode. The flow rate was 1.0 mL x min(-1), detection wavelengthes were 345 nm for jatrorrhizine , palmatine, berberine and 210 nm for obacunone.</p><p><b>RESULT</b>The average recoveries of jatrorrhizine, palmatine, berberine, and obacunone were 98.94%, 101.17%, 96.22% and 98.90%, respectively.</p><p><b>CONCLUSION</b>The method is simple, accurate and repeatable, and can be used in content determination of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Benzoxepins / Berberine / Berberine Alkaloids / Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Phellodendron / Limonins Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2010 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Benzoxepins / Berberine / Berberine Alkaloids / Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Phellodendron / Limonins Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2010 Type: Article