Expression of pituitary tumor-transforming gene 1 during the development of androgen-independent prostate cancer / 中华男科学杂志
National Journal of Andrology
;
(12): 686-691, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-262324
ABSTRACT
<p><b>Objective</b>To explore the expression of pituitary tumor transforming gene 1 (PTTG1) during the transformation of prostate cancer from androgen-dependent (ADPC) to androgen-independent (AIPC).</p><p><b>METHODS</b>We established an AIPC cell model LNCaP-AI by culturing the androgen-dependent LNCaP cell line in the hormone-deprived medium for over 3 months. The cell model was verified and the PTTG1 expression in the LNCaP cells was detected by Western blot and RT-PCR during hormone deprivation.</p><p><b>RESULTS</b>The AIPC cell model LNCaP-AI was successfully established. The PTTG1 expression was gradually increased in the LNCaP cells with the prolonged time of hormone deprivation and the expressions of matrix metalloproteinases MMP-2 and -9 were elevated at the same time.</p><p><b>CONCLUSIONS</b>The expression of PTTG1 is increased gradually in AIPC, which may be a target of gene therapy for advanced prostate cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prostatic Neoplasms
/
Gene Expression Regulation, Neoplastic
/
Blotting, Western
/
Matrix Metalloproteinase 2
/
Matrix Metalloproteinase 9
/
Cell Line, Tumor
/
Securin
/
Genetics
/
Metabolism
/
Neoplasms, Hormone-Dependent
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2016
Type:
Article
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