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Kidney tubular-cell secretion of osteoblast growth factor is increased by kaempferol: a scientific basis for "the kidney controlling the bone" theory of Chinese medicine / 中国结合医学杂志
Chinese journal of integrative medicine ; (12): 675-681, 2014.
Article in English | WPRIM | ID: wpr-262631
ABSTRACT
<p><b>OBJECTIVE</b>To study, at the cytological level, the basic concept of Chinese medicine that "the Kidney (Shen) controls the bone".</p><p><b>METHODS</b>Kaempferol was isolated form Rhizoma Drynariae (Gu Sui Bu, GSB) and at several concentrations was incubated with opossum kidney (OK) cells, osteoblasts (MC3T3 E1) and human fibroblasts (HF) at cell concentrations of 2×10(4)/mL. Opossum kidney cell-conditioned culture media with kaempferol at 70 nmol/L (70kaeOKM) and without kaempferol (0OKM) were used to stimulate MC3T3 E1 and HF proliferation. The bone morphological protein receptors I and II (BMPR I and II) in OK cells were identified by immune-fluorescence staining and Western blot analysis.</p><p><b>RESULTS</b>Kaempferol was found to increase OK cell growth (P<0.05), but alone did not promote MC3T3 E1 or HF cell proliferation. However, although OKM by itself increased MC3T3 E1 growth by 198% (P<0.01), the 70kaeOKM further increased the growth of these cells by an additional 127% (P<0.01). It indicates that the kidney cell generates a previously unknown osteoblast growth factor (OGF) and kaempferol increases kidney cell secretion of OGF. Neither of these media had any significant effect on HF growth. Kaempferol also was found to increase the level of the BMPR II in OK cells.</p><p><b>CONCLUSIONS</b>This lends strong support to the original idea that the Kidney has a significant influence over bone-formation, as suggested by some long-standing Chinese medical beliefs, kaempferol may also serve to stimulate kidney repair and indirectly stimulate bone formation.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Opossums / Osteoblasts / Pharmacology / Physiology / Cell Line / Chemistry / 3T3 Cells / Culture Media, Conditioned / Bodily Secretions / Intercellular Signaling Peptides and Proteins Type of study: Diagnostic study / Prognostic study Limits: Animals Language: English Journal: Chinese journal of integrative medicine Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Opossums / Osteoblasts / Pharmacology / Physiology / Cell Line / Chemistry / 3T3 Cells / Culture Media, Conditioned / Bodily Secretions / Intercellular Signaling Peptides and Proteins Type of study: Diagnostic study / Prognostic study Limits: Animals Language: English Journal: Chinese journal of integrative medicine Year: 2014 Type: Article