Effects of 5-hydroxymethyl furfural extracted from Rehmannia glutinosa Libosch on the expression of signaling molecules relevant to learning and memory among hippocampal neurons exposed to high concentration of corticosterone / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 844-849, 2014.
Article
in English
| WPRIM
| ID: wpr-262704
ABSTRACT
<p><b>OBJECTIVE</b>To determine the effects of 5-hydroxymethyl furfural (5-HMF), an extract of Rehmannia glutinosa Libosch, on several down-regulated signaling molecules involved in learning and memory in hippocampal neurons.</p><p><b>METHODS</b>After cultured for 7 days, primary hippocampal neurons were divided into 5 groups normal, corticosterone model, RU38486, 5-HMF, and donepezil group. Neuron survival rates were calculated 24 h later using SYTO13-PI double-fluorescence staining and an 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. β-galactosidase activity was also assayed. Protein expressed by the glucocorticoid receptor (GCR), brainderived neurotrophic factor (BDNF), and N-methyl-D-aspartate receptor 2B (NR2B), as well as phosphorylationcyclic adenosine monophosphate (cAMP) response element binding protein (p-CREB), phosphorylation-extracellular signal-regulated kinase (p-ERK), and phosphorylation-synapsin (p-synapsin) were quantified with Western blot.</p><p><b>RESULTS</b>Hippocampal neuron survival rates and the above-mentioned proteins were dramatically decreased (P<0.05), β-galactosidase activity was significantly increased in the model group. but the effect was reversed by 5-HMF, RU38486, and to a lesser extent by donepezil (P<0.05).</p><p><b>CONCLUSION</b>5-HMF extracts from the Chinese herb Rehmannia glutinosa Libosch could protect hippocampal neurons from glucocorticoid injury and from down-regulated signaling molecules in the GCR-BDNF-NR2B-p-ERK-p-CREB-p-synapsin signal transduction pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Corticosterone
/
Signal Transduction
/
Chemistry
/
Blotting, Western
/
Rats, Sprague-Dawley
/
Cell Biology
/
Rehmannia
/
Furaldehyde
/
Hippocampus
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Chinese journal of integrative medicine
Year:
2014
Type:
Article
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