Your browser doesn't support javascript.
loading
Effect of protein kinase C and calcium on the thrombin receptors activation / 中华血液学杂志
Chinese Journal of Hematology ; (12): 154-157, 2008.
Article in Chinese | WPRIM | ID: wpr-262916
ABSTRACT
<p><b>OBJECTIVE</b>To compare the effects of protein kinase C (PKC) and calcium (Ca2+) on platelet aggregation and platelet membrane surface GP I b expression in thrombin receptors activation, and investigate the role of Gq signal transduction pathway in such activation.</p><p><b>METHODS</b>Peptide SFLLRN (PARI-AP) and AYPGKF (PAR4-AP) were used to stimulate platelet, and the effects of Ro-31-2220 (inhibitor of PKC) and BAPTA/AM (calcium chelator) on the platelet aggregation and GP I b were analyzed.</p><p><b>RESULTS</b>Either 25 micromol/L PAR1 or 250 micromol/L PAR4 peptide could induce absolute platelet aggregation with a reversible internalization of GP I b. Platelet aggregation was inhibited by Ro-31-2220 or BAPTA while the morphological change curve still occurred upon PARs activation. In addition, Ro-31-2220 decreased GP I b centralization upon PAR1 stimulation [(87.00 +/- 0.04)% and (73.00 +/- 0.08)%, respectively at 1, 2 min, P<0.05], albeit it blocked the internalization of GP I b in PAR4 activation [(44.00 +/- 0.01)% and (46.00 +/- 0.05)%, respectively at 10, 30 min, P <0.05]. Meanwhile, GP I b internalization was blocked by BAPTA in both peptides [(94.00 +/- 0.08)% and (95.00 +/- 0.00)% at 1 min, (92.00 +/- 0.02)% and (94.00 +/- 0.01)% at 2 min, (91.00 +/- 0.02)% and (91.00 +/- 0.02)% at 5 min, (90.00 +/- 0.04)% and (87.00 +/- 0.03)% at 10 min, respectively, P <0.05].</p><p><b>CONCLUSION</b>PKC and calcium play an important role in thrombin receptor activation. Calcium is closely correlated with such activation, being similar in the two PARs signal pathways. PK C promotes GP I b centralization in PAR1 pathway and accelerates GP I b return to membrane surface in PAR4 pathway.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Blood Platelets / Protein Kinase C / Signal Transduction / Platelet Aggregation / Calcium / Receptors, Thrombin / Platelet Glycoprotein GPIb-IX Complex / Metabolism Limits: Adult / Female / Humans / Male Language: Chinese Journal: Chinese Journal of Hematology Year: 2008 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Blood Platelets / Protein Kinase C / Signal Transduction / Platelet Aggregation / Calcium / Receptors, Thrombin / Platelet Glycoprotein GPIb-IX Complex / Metabolism Limits: Adult / Female / Humans / Male Language: Chinese Journal: Chinese Journal of Hematology Year: 2008 Type: Article