Inhibition of tissue factor by siRNA enhances doxorubicin-induced apoptosis in human neuroblastoma / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 594-597, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-262978
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the regulation of tissue factor (TF) on doxorubicin-induced apoptosis in human neuroblastoma.</p><p><b>METHOD</b>The expression of TF was examined by Western blotting. TF siRNA-pSUPER plasmid was constructed by inserting a specific 19-nt silencing sequence targeting TF gene into pSUPER vector. Transfection of TF siRNA-pSUPER was performed using lipofectamine 2000. The activation of caspase-3 and PARP induced by doxorubicin was tested by Western blotting. The apoptotic cells were stained by Hochest 33342 and counted under fluorescence inverted microscope.</p><p><b>RESULTS</b>(1) Human neuroblastoma cell line SK-N-MC expressed high level of TF. (2) Downregulation of TF expression was achieved by transfection of TF siRNA-pSUPER into SK-N-MC cells in a dose-dependent manner. (3) Cleavage of caspase-3 and PARP was increased in transfected SK-N-MC cell with down-regulation of TF. (4) TF siRNA treatment at 1 microg/ml for 8 h significantly increased apoptotic cell number in transfected SK-N-MC cells compared to that in non-transfected cells (P < 0.05) while exposing to 1 microg/ml doxorubicin for 8 h.</p><p><b>CONCLUSIONS</b>Downregulation of TF expression by specific siRNA vector could increase the cytotoxicity of doxorubicin and enhance doxorubicin-induced apoptosis in human neuroblastoma cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Thromboplastin
/
Transfection
/
Doxorubicin
/
Poly(ADP-ribose) Polymerases
/
Apoptosis
/
RNA, Small Interfering
/
RNA Interference
/
Cell Line, Tumor
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2007
Type:
Article
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