Effects of borneol on pharmacokinetics of protocatechuic acid in rabbits / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of borneol on the pharmacokinetics of protocatechuic acid (PA) in rabbits.</p><p><b>METHOD</b>A method for determinating PA in rabbit plasma by high performance liquid chromatography (HPLC) was established. The 12 healthy New Zealand rabbits were randomly divided into 2 groups. PA concentration in plasma samples of rabbits was determined by HPLC after oral administration of PA (30 mg x kg(-1)) and PA combined with borneol (protocatechuic acid 30 mg x kg(-1) + borneol 15 mg x kg(-1)). The data were processed by DAS 2.0 software to calculate the pharmacokinetic parametes.</p><p><b>RESULT</b>Chromatographic peaks were separated well by the HPLC method. Regression analysis of the data of PA concentration against its peak area showed a good straight line in the range of 0.04-2.0 mg x L(-1) (r = 0.9983). In comparison with PA alone, the pharmacokinetics parameters of PA (t1/2Ka, T(max), V1/F, CL/F) in the PA + borneol combination group were decreased, while the AUC(0-infinity) was increased.</p><p><b>CONCLUSION</b>Borneol could significantly promote absorption, increase the dose absorption and slow elimination of PA in rabbits. Borneol could improve the bioavailability of PA.</p>