Simultaneous determination of three coumarin constituents in roots of Peucedanum praeruptorum by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1121-1123, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-263028
ABSTRACT
<p><b>OBJECTIVE</b>To develop a reserved-phase HPLC method for the determination of praeruptorin A, praeruptorin B, qianhucoumarin E in roots of Peucedanum praeruptorum.</p><p><b>METHOD</b>Agilent TC-C18 column (4.6 mm x 250 mm, 5 microm) was used at 30 degrees C with the mobile phase of methanol-water (7525). The flow rate was set at 0.8 mL x min(-1). The detection wavelength was 321 nm.</p><p><b>RESULT</b>The linear response ranged from 3.20-28.80 microg for +/- praeruptorin A (r = 0.9999, n = 5), 1.60-14.40 g for praeruptorin B (r = 0.9995, n = 5) and 1.64-14.76 g for qianhucoumarin E (r = 0.9994, n = 5), respectively. Recoveries were 98.92% with RSD 1.6% for praeruptorin A, 99.66% with RSD 1.5% for praeruptorin B and 99.72% with RSD 1.4% for qianhucoumarin E.</p><p><b>CONCLUSION</b>The method is quick, simple and repeatable for determination of three coumarin constituents in root of P. praeruptorum.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Linear Models
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Plant Roots
/
Apiaceae
/
Coumarins
Type of study:
Prognostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2009
Type:
Article
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