Effect of 5F from Pteris semipinnata on expression of Nr1d1 in HO-8910PM cell line / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1268-1271, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-263052
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of PsL5F (ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid, an extract from Pteris semipinnata L) on the expression of nuclear receptor subfamily 1, group D, member 1 (Nr1d1) in highly metastatic ovarian carcinoma HO-8910PM cells, and its mechanisms.</p><p><b>METHOD</b>Microarray Chip was used to examine the level of Nr1d1 mRNA expression on HO-8910PM cells treated with PsL5F. Fluorescent quantitative real-time PCR assay and Western blot were performed to verify the effects of PsL5F on Nr1d1 mRNA and protein expression.</p><p><b>RESULT</b>After 24 h treatment of 100 micromol x L(-1) PsL5F, the mRNA and protein levels of Nr1d1 in HO-8910PM cells were 35.34 +/- 1.07 and 7.71 +/- 0.43 times compared to those of control group (P < 0.01, P < 0.01), respectively.</p><p><b>CONCLUSION</b>PsL5F can up-regulate significantly the expression of Nr1d1 in HO-8910PM cells. Antitumor effects and its mechanisms of PsL5F in HO-8910PM cells may be involved in the up-regulation of Nr1d1 expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovarian Neoplasms
/
Pathology
/
Pharmacology
/
Piperidones
/
RNA, Messenger
/
Drug Screening Assays, Antitumor
/
Gene Expression
/
Chemistry
/
Pteris
/
Cell Line, Tumor
Limits:
Female
/
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2009
Type:
Article
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