Suppressive effect of resveratrol on growth of U251 human glioma cells and its correlated mechanism / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1027-1031, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-263074
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the suppressive effect of resveratrol on growth of U251 human glioma cells and its correlated mechanism.</p><p><b>METHOD</b>U251 human glioma cells were treated with resveratrol at various concentrations, MTT assay was used to determine the inhibitory rate of cell proliferation, FCM to detect the cell apoptosis, the expressions of Bcl-2, Bcl-XL, STAT3 and CyclinD1 were analysed by immunohistochemistry and Western blot to examine the expression of Bcl-2, Bcl-XL, STAT3, CyclinD1, Caspase-3 and Bax.</p><p><b>RESULT</b>After treatment with resveratrol, MTT assay showed the growth of U251 cells was inhibited in dose-dependent and time-dependent manners, apoptosis of cells advanced stage was built up, immunohistochemical staining displayed decreased the expression of Bcl-2, Bcl-XL, STAT3 and CyclinD1 and Western blot showed that resveratrol decreased the expression of Bcl-2, Bcl-XL, STAT3 and CyclinD1, and built up Bax and Caspase-3.</p><p><b>CONCLUSION</b>It is possible that downregulated the expression of Bcl-2, Bcl-XL, but upregulated Bax and Caspase-3, and the indication was obviously in dose-dependent and time-dependent manners.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stilbenes
/
Immunohistochemistry
/
Gene Expression Regulation, Neoplastic
/
Blotting, Western
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Cyclin D1
/
Cell Line, Tumor
/
Therapeutic Uses
/
Drug Therapy
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2009
Type:
Article
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