Promoter trapping in Magnaporthe grisea / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 28-33, 2006.
Article
in English
| WPRIM
| ID: wpr-263228
ABSTRACT
Application of promoter trapping based on transformation in Magnaporthe grisea is reported in this paper. Two promoter-trapping vectors, designated as pCBGFP and pEGFPHPH, were constructed and transformed into protoplasts of M. grisea. A library of 1,077 transformants resistant to hygromycin B was generated. Of which, 448 transformants were found to express eGFP gene in different structures of M. grisea. Three transformants grew slowly, 5 transformants decreased in conidiation and 7 transformants reduced in pathogenicity greatly among these 448 transformants. Eleven transformants were checked by genomic southern blot randomly, and 9 of which were single-copy insertions. The promoter trapping technique has been applied successfully in M. grisea and can be used as a tool for functional genomic analysis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Proteins
/
Fungal Proteins
/
Protein Engineering
/
Gene Expression Regulation, Fungal
/
Promoter Regions, Genetic
/
Genes, Reporter
/
Magnaporthe
/
Green Fluorescent Proteins
/
Genetic Vectors
/
Genetics
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2006
Type:
Article
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