Effects of baicalin on the expression of pro-MMP-1 and MMP-3 in human gingival fibroblasts and periodontal ligament cells / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 197-200, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-263417
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of baicalin on the IL-1beta induced pro-MMP-1 in HGF and the effects of baicalin on MMP-3 expression in periodontal ligament cells (PDLCs).</p><p><b>METHODS</b>The amount of secreted pro-MMP-1 and MMP-3 expression was detected by ELISA and cell immunochemistry.</p><p><b>RESULTS</b>(1) The amount of secreted pro-MMP-1 (3.333 +/- 0.123) microg/L increased significantly following 1 microg/L of IL-1beta, compared with control group (1.960 +/- 0.180) microg/L. Addition of baicalin to cell culture medium for 1 hour following IL-1beta decreased pro-MMP-1 secretion in a dose-dependent manner in the range of 10 approximately 1,000 microg/L. (2) 1 microg/L IL-1beta could significantly stimulate the synthesis and secretion of MMP-3 in PDLCs. (3) The baicalin could not interfere the synthesis of MMP-3, but could inhibit the release of MMP-3 from PDLCs.</p><p><b>CONCLUSIONS</b>Baicalin could inhibit the secretion of pro-MMP-1 and MMP-3 expression in IL-1beta induced HGF and PDLCs, which suggests that baicalin may play an important role in preventing and treating periodontal disease.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Peptide Fragments
/
Periodontal Ligament
/
Periodontitis
/
Pharmacology
/
Flavonoids
/
Metalloendopeptidases
/
Chemistry
/
Interleukin-1
/
Collagenases
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2004
Type:
Article
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