Bioassay of the soluble human tumor necrosis factor receptor I recombinant plasmid expression in vitro / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 185-188, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-263420
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of recombinant plasmid PcDNA3.1-sTNFRI in vitro and evaluate the bioactivity of expressed sTNFRI.</p><p><b>METHODS</b>CHO cells were transfected with recombinant plasmid PcDNA3.1-sTNFRI by liposome. sTNFRI in cell culture supernatant was detected by ELISA and sTNFRI blockage of TNF-alpha cytotoxicity in L929 cells evaluated by MTT assay.</p><p><b>RESULTS</b>The expression of sTNFRI in transfected cell culture supernatant was higher than control groups (P < 0.001). The expressed sTNFRI could significantly neutralize TNF-alpha cytotoxicity in L929 cells.</p><p><b>CONCLUSIONS</b>These results showed that the recombinant plasmid PcDNA3.1-sTNFRI can be expressed in mammalian cells and the recombinant sTNFRI has biological function.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plasmids
/
Recombinant Fusion Proteins
/
Transfection
/
CHO Cells
/
Cloning, Molecular
/
DNA, Complementary
/
Receptors, Tumor Necrosis Factor, Type I
/
Eukaryotic Cells
/
Genetic Vectors
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2004
Type:
Article
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