Cloning of AE1-c-end cDNA and construction of its expression plasmid for yeast two-hybrid system / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 284-290, 2002.
Article
in Zh
| WPRIM
| ID: wpr-263608
Responsible library:
WPRO
ABSTRACT
In this study, about 350 bp cDNA fragment was amplified by PCR. After being sequenced, the AE1-c-end gene fragment was cloned into EcoR I-Pst I site of pGADT7 to form AD ends in the yeast two-hybrid system. The recombinant plasmid was transformed into yeast AH109, and the expression in the yeast was observed. The results demonstrate that AE1-c-end was obtained. pGADT7-AE1-c-end has no toxic effect on the yeast. It can serve as a target gene of yeast two-hybrid system.
Full text:
1
Index:
WPRIM
Main subject:
Plasmids
/
Yeasts
/
Polymerase Chain Reaction
/
Cloning, Molecular
/
DNA, Complementary
/
Two-Hybrid System Techniques
Language:
Zh
Journal:
Journal of Biomedical Engineering
Year:
2002
Type:
Article