Analysis of differentiation characters in osteosarcoma Mdr sublines / 中国骨伤
China Journal of Orthopaedics and Traumatology
;
(12): 368-372, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-263683
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between multidrug resistance (Mdr) and malignancy. To observe whether P-glycoprotein (P-gp) overexpression had the same bioactivity as osteogenic stem cell turning into more mature cell or more complex phenotype when parent cell line turned to Mdr phenotype.</p><p><b>METHODS</b>Six cell sublines of Mdr phenotype with different expression degree were analyzed. Stathmin generally identified in malignancy cell and stem cell, was a microtubule associated protein and the signal of differentiation in osteogenic stem cell. RT-PCR and hybridization in situ were used to analyze the relationship between Mdr1 mRNA and expression of Stathmin mRNA and VEGF mRNA.</p><p><b>RESULTS</b>Morphological and functional analysis of Mdr phenotype showed the P-gp-positive cell lines were more differentiated than the parent cells in terms of enhanced activity of cellular alkaline phosphatase. These subclones all displayed a decrease in potential malignancy such as tumor growth rate and metastatic ability. A significant negative correlation could be identified between Mdr1 mRNA and expression of VEGF and Stathmin mRNA.</p><p><b>CONCLUSION</b>Expression of Mdr1/P170 indicated osteosarcoma cells differentiated towards more mature state, which will develop the new research field of Mdr and supply the new research method of the function of P- gp.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteosarcoma
/
Gene Expression
/
Cell Differentiation
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
Multidrug Resistance-Associated Proteins
/
Cell Line, Tumor
/
Vascular Endothelial Growth Factor A
/
Genetics
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Orthopaedics and Traumatology
Year:
2008
Type:
Article
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