HLA-B gene polymorphism detected by high-resolution sequence-based typing in Guangdong Han populations / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 50-54, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-263854
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the genetic polymorphism of HLA-B locus in Guangdong Han population and compare the characteristic of the allele frequency distribution with that in other populations.</p><p><b>METHODS</b>A total of 562 cord blood samples from Guangzhou Cord Blood Bank were analyzed by sequence-based typing. Then the sequences encompassing exons 2, 3, and 4 for HLA-B gene were analyzed by direct sequencing of PCR products. The allele frequency distribution of HLA-B in this population was compared with that in other populations.</p><p><b>RESULTS</b>A total of 59 different HLA-B alleles were detected, and among them were 6 HLA-B alleles with frequencies higher than 5% HLA-B*4601 (14.5%), HLA-B*400101 (14.4%), HLA-B*1502 (11.5%), HLA-B*1301 (8.6%), HLA-B*5801 (8.1%) and HLA-B*380201 (6.4%); the total frequency of these six alleles was 63.5%. At the same time, there were 30 kinds of HLA-B allele with frequencies lower than 0.5%; the total frequency of these alleles was 4.9%. Maximum variation at HLA-B was seen in the HLA-B*15 allele family (nine alleles). Comparison of the HLA-B frequencies in different populations showed a close relationship of Guandong Han population with the Chinese populations from Hong Kong and Singapore, respectively.</p><p><b>CONCLUSION</b>The results have shown the characteristic of HLA-B distribution and provided more accurate genotypic data that may serve as normal reference value for the Han population in Guangdong, China.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Genetic
/
HLA-B Antigens
/
Ethnicity
/
China
/
Asian People
/
Ethnology
/
Gene Frequency
/
Genetics
/
Genetics, Population
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2006
Type:
Article
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