Genetic polymorphism of 6 short-tandem repeat loci in Miao minority group of Rongshui county in Guangxi province / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 709-711, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-263866
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the distributions of six short-tandem repeat (STR) loci, namely D7S820, D13S317, D16S539, HUMCSF1PO, HUMTPOX and HUMTH01, in Miao minority group at Rongshui county in Guangxi province and construct the relevant genetic database.</p><p><b>METHODS</b>Sodium-citrated blood specimens were collected from 208 healthy unrelated Miao individuals in Rongshui county. The DNAs from the specimens were extracted with phenol-chloroform method; AmplFSTR Identifier PCR Amplification Kit was used to amplify the extracted DNAs, and 3100 Genetic Analyzer was used to analyze and screen the amplified products.</p><p><b>RESULTS</b>In this study, 7, 8, 6, 7, 5, 7 alleles were observed at the 6 STR loci respectively. The expected distribution of genotype accorded with Hardy-Weinbery equilibrium. The total discrimination power, cumulative paternity exclusion power and total polymorphism information were 0.999995, 0.9959 and 0.9987 respectively.</p><p><b>CONCLUSION</b>The results demonstrate that these 6 STR loci are of high polymorphism and hereditary stability and are in accord with Mendel's law. The data obtained are valuable in population genetics research, forensic application, and individual identifications.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Genetic
/
Ethnicity
/
Linkage Disequilibrium
/
China
/
Polymerase Chain Reaction
/
Microsatellite Repeats
/
Gene Frequency
/
Genetics
Limits:
Adolescent
/
Child
/
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2005
Type:
Article
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