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A method for combining Fluoro-Jade B staining and immunofluorescent staining / 南方医科大学学报
Journal of Southern Medical University ; (12): 671-674, 2016.
Article in Chinese | WPRIM | ID: wpr-263983
ABSTRACT
<p><b>OBJECTIVE</b>To explore a method for combining Fluoro-Jade B (FJB) staining with immunofluorescent staining in rats with focal cortical infarction.</p><p><b>METHOD</b>Permanent distal middle cerebral artery occlusion (dMCAO) was induced in rats by electrocoagulation. The rat models were randomized into two groups, and frozen sections of the brain tissues from each group were stained with FJB followed by immunofluorescent staining or in the reverse order.</p><p><b>RESULTS</b>FJB staining followed by immunofluorescence staining clearly visualized both FJB-positive and immunofluorescence-positive cells in the frozen sections, but the staining protocol in the reverse sequence failed to clearly show the immunofluorescence-positive cells.</p><p><b>CONCLUSION</b>FJB staining prior to immunofluorescence staining does not affect the staining effect of protein immunofluorescent staining and better visualizes the positive cells.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Staining and Labeling / Brain / Chemistry / Fluorescent Antibody Technique / Infarction, Middle Cerebral Artery / Fluoresceins / Fluorescent Dyes / Methods Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Staining and Labeling / Brain / Chemistry / Fluorescent Antibody Technique / Infarction, Middle Cerebral Artery / Fluoresceins / Fluorescent Dyes / Methods Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2016 Type: Article