Overexpression of Axin inhibits lymphoma cell invasion and metastasis in vitro by down-regulating β-catenin and MMP7/MMP9 / 南方医科大学学报
Journal of Southern Medical University
;
(12): 423-428, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-264027
ABSTRACT
<p><b>OBJECTIVE</b>To investigate that the role of Axin in regulating the invasion and migration ability of lymphoma cells and explore the molecular mechanisms.</p><p><b>METHODS</b>The expressions of Axin, β-catenin, MMP7, and MMP9 were detected in different lymphoma cell lines by RT-PCR and Western blotting. A lymphoma cell line with low Axin expressions was transiently transfected with pCMV5-HA-Axin and pcDNA5-His-β-catenin plasmid, and the expressions of β-catenin, MMP7, and MMP9 mRNA and protein were observed. A lymphoma cell model stably overexpressing Axin was transfected with AXIN-shRNA and β-catenin-shRNA, and the changes in β-catenin, MMP7, and MMP9 cexpressions were observed. The changes in the invasion and migration abilities of this cell model were assessed following Axin knockdown.</p><p><b>RESULTS</b>In the lymphoma cell lines tested, the Axin expression showed a negative correlation with β-catenin, MMP7, and MMP9 expressions. In Raji cells with a low Axin expression, overexpression of Axin resulted in decreased expressions of β-catenin, MMP7, and MMP9 at the protein levels but not the mRNA levels, and overexpression of β-catenin obviously increased MMP7 and MMP9 mRNA and protein expressions. In the cells with stable Axin overexpression, Axin knockdown caused increased expressions of β-catenin, MMP7, and MMP9 at the protein levels but not the mRNA levels, while β-catenin knockdown caused lowered expressions of MMP7 and MMP9 and suppressed cell invasion and migration.</p><p><b>CONCLUSION</b>In lymphoma cells, Axin overexpression can decrease the expression of β-catenin, which in turn decreases the expressions of MMP7 and MMP9 to inhibit the cell invasion and migration.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
RNA, Messenger
/
Transfection
/
Down-Regulation
/
Matrix Metalloproteinase 9
/
Matrix Metalloproteinase 7
/
RNA, Small Interfering
/
Cell Line, Tumor
/
Beta Catenin
/
Gene Knockdown Techniques
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS