Fabrication of a new composite scaffold material for delivering rifampicin and its sustained drug release in rats / 南方医科大学学报
Journal of Southern Medical University
; (12): 309-315, 2016.
Article
in Zh
| WPRIM
| ID: wpr-264049
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To fabricate a new composite scaffold material as an implant for sustained delivery of rifampicin and evaluate its performance of sustained drug release and biocompatibility.</p><p><b>METHODS</b>The composite scaffold material was prepared by loading poly(lactic-co-glycolic) acid (PLGA) microspheres that encapsulated rifampicin in a biphasic calcium composite material with a negative surface charge. The in vitro drug release characteristics of the microspheres and the composite scaffold material were evaluated; the in vivo drug release profile of the composite scaffold material implanted in a rat muscle pouch was evaluated using high-performance liquid chromatography. The biochemical parameters of the serum and liver histopathologies of the rats receiving the transplantation were observed to assess the biocompatibility of the composite scaffold material.</p><p><b>RESULTS</b>The encapsulation efficiency and drug loading efficiency of microspheres were (56.05±5.33)% and (29.80±2.88)%, respectively. The cumulative drug release rate of the microspheres in vitro was (94.19±5.4)% at 28 days, as compared with the rate of (82.23±6.28)% of composite scaffold material. The drug-loaded composite scaffold material showed a good performance of in vivo drug release in rats, and the local drug concentration still reached 16.18±0.35 µg/g at 28 days after implantation. Implantation of the composite scaffold material resulted in transient and reversible liver injury, which was fully reparred at 28 days after the implantation.</p><p><b>CONCLUSION</b>The composite scaffold material possesses a good sustained drug release capacity and a good biocompatibility, and can serve as an alternative approach to conventional antituberculous chemotherapy.</p>
Full text:
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Index:
WPRIM
Main subject:
Polyglycolic Acid
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Rifampin
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Biocompatible Materials
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Drug Carriers
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Chemistry
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Lactic Acid
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Delayed-Action Preparations
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Drug Liberation
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Microspheres
Limits:
Animals
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2016
Type:
Article