A preliminary analysis of non-small cell lung cancer biomarkers in serum / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 140-148, 2003.
Article
in English
| WPRIM
| ID: wpr-264283
ABSTRACT
<p><b>OBJECTIVE</b>To identify potential serum biomarkers that could be used to discriminate lung cancers from normal.</p><p><b>METHODS</b>Proteomic spectra of twenty-eight serum samples from patients with non-small cell lung cancer and twelve from normal individuals were generated by SELDI (Surfaced Enhanced Laser Desorption/Ionization) Mass Spectrometry. Anion-exchange columns were used to fractionate the sera into 6 designated pH groups. Two different types of protein chip arrays, IMAC-Cu and WCX2, were employed. Samples were examined in PBSII Protein Chip Reader (Ciphergen Biosystem Inc) and the discriminatory profiling between cancer and normal samples was analyzed with Biomarker Pattern software.</p><p><b>RESULTS</b>Five distinct potential lung cancer biomarkers with higher sensitivity and specificity were found, with four common biomarkers in both IMAC-Cu and WCX2 chip; the remaining biomarker occurred only in WCX2 chip. Two biomarkers were up-regulated while three biomarkers were down-regulated in the serum samples from patients with non-small cell lung cancer. The sensitivities provided by the individual biomarkers were 75%-96.43% and specificities were 75%-100%.</p><p><b>CONCLUSIONS</b>The preliminary results suggest that serum is a capable resource for detecting specific non-small cell lung cancer biomarkers. SELDI mass spectrometry is a useful tool for the detection and identification of new potential biomarker of non-small cell lung cancer in serum.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Biomarkers, Tumor
/
Sensitivity and Specificity
/
Carcinoma, Non-Small-Cell Lung
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Diagnosis
/
Lung Neoplasms
Type of study:
Diagnostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2003
Type:
Article
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