Studies on purification of methamidophos monoclonal antibodies and comparative immunoactivity of purified antibodies / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 119-125, 2003.
Article
in English
| WPRIM
| ID: wpr-264286
ABSTRACT
<p><b>OBJECTIVE</b>To purify Methamidophos (Met) monoclonal antibodies with two methods and compare immune activity of purified antibodies.</p><p><b>METHOD</b>Caprylic acid ammonium sulphate precipition (CAASP) method and Sepharose protein-A (SPA) affinity chromatography method were used to purify Met monoclonal antibodies, UV spectrum scanning was used to determine protein content and recovery of purified antibodies, sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze the purity of purified antibodies, and enzyme-linked immunosorbent assay (ELISA) was used to determine immune activity of purified antibodies.</p><p><b>RESULTS</b>Antibody protein content and recovery rate with CAASP method were 7.62 mg/mL and 8.05% respectively, antibody protein content and recovery rate with SPA method were 6.45 mg/mL and 5.52% respectively. Purity of antibodies purified by SPA method was higher than that by CAASP method. The half-maximal inhibition concentration (IC50) of antibodies purified by SPA to Met was 181.26 microg/mL, and the linear working range and the limit of quantification (LOD) were 2.43-3896.01 microg/mL and 1.03 microg/mL, respectively. The IC50 of antibodies purified by CAASP to Met was 352.82 microg/mL, and the linear working range and LOD were 10.91-11412.29 microg/mL and 3.42 microg/mL, respectively.</p><p><b>CONCLUSION</b>Antibodies purified by SPA method are better than those by CAASP method, and Met monoclonal antibodies purified by SPA method can be used to prepare gold-labelled testing paper for analyzing Met residue in vegetable and drink water.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organothiophosphorus Compounds
/
Vegetables
/
Enzyme-Linked Immunosorbent Assay
/
Pesticide Residues
/
Food Contamination
/
Chromatography, Affinity
/
Chromatography, Agarose
/
Electrophoresis, Polyacrylamide Gel
/
Allergy and Immunology
/
Fruit
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2003
Type:
Article
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