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Determination of organic acids in dental plaque with high performance liquid chromatography / 华西口腔医学杂志
Article in Chinese | WPRIM | ID: wpr-264386
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish determination method of formic acid, lactic acid, acetic acid and succinic acid in dental plaque with high performance liquid chromatography (HPLC).</p><p><b>METHODS</b>After the samples were centrifuged, 2 microL supernatant was transferred to a 1 mL centrifuge tube and diluted in water, then was determined with HPLC. The mixture of phosphate buffer and methanol (973) as mobile phase throughout the experiment. The determination of organic acid was performed on Phenomenex C18 column and at their maximum absorption wave.</p><p><b>RESULTS</b>The linear ranges of formic acid, lactic acid, acetic acid and succinic acid were 0.110-500, 0.049-500, 0.047-500, 0.084-500 microg/mL. The detection limits were 0.110, 0.049, 0.047, 0.084 microg/mL. The relative standard derivation were 9.5%, 7.9%, 4.3%, 4.2%. The average recoveries of samples were 82%-112%, 82%-102.5%, 90%-115%, 80%-110%.</p><p><b>CONCLUSION</b>The method was simple, quick and adapt for analysis of organic acid in dental plaque.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Chromatography, High Pressure Liquid / Dental Plaque / Formates Language: Chinese Journal: West China Journal of Stomatology Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Chromatography, High Pressure Liquid / Dental Plaque / Formates Language: Chinese Journal: West China Journal of Stomatology Year: 2008 Type: Article