Effect of salidroside on salivary adenoid cystic carcinoma cells in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 312-319, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-264430
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect and mechanism of salidroside on salivary adenoid cystic carcinoma (SACC-2) cells in vitro.</p><p><b>METHODS</b>To detect the effect of salidroside on SACC-2 cells growth with CCK-8 kit, the growth curve of cells were drawn. To detect the expression of cysteine proteinase (Caspase 3 and Caspase 8) and proliferating cell nuclear antigen (PCNA) in SACC-2 cells, immunohistochemistry staining was used.</p><p><b>RESULTS</b>According the results of CCK-8 kit detected, salidroside could inhibit the proliferation of SACC-2 cells, IC50 value was (4.99+/-0.23) microg/mL. Growing curve showed that SACC-2 cells of salidroside groups decreased with extending cell culture time. Immunohistochemistry staining showed that Caspase 3 and Caspase 8 were both strong positive expression in SACC-2 cells of salidroside groups, and poor positive expression in SACC-2 cells of control group, the difference was significant (P<0.01). The expression of PCNA was reverse (P<0.01).</p><p><b>CONCLUSION</b>Salidroside could inhibit the proliferation of SACC-2 cells and induce SACC-2 cell apoptosis in vitro, which could be a kind of antitumor medicine in the future.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenols
/
In Vitro Techniques
/
Salivary Gland Neoplasms
/
Immunohistochemistry
/
Apoptosis
/
Carcinoma, Adenoid Cystic
/
Cell Line, Tumor
/
Cell Proliferation
/
Glucosides
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2008
Type:
Article
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