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Downregulation of transforming growth factor-beta1 and platelet-derived growth factor gene expression by interleukin-10 in murine hepatic stellate cells in response to experimental liver injury / 中华外科杂志
Chinese Journal of Surgery ; (12): 282-286, 2005.
Article in Chinese | WPRIM | ID: wpr-264524
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of interleukin-10 (IL-10) on the expression of transforming growth factor-beta(1) (TGFbeta(1)) and platelet-derived growth factor (PDGF) in hepatic stellate cells (HSC) during liver injury.</p><p><b>METHODS</b>The adenovirus vector (the titer was 1 x 10(7) efu/ml) encoded IL-10 gene was used to transfect the rat via the vein of caudal. At the same time, CCl(4) was injected into rat by a hypodermic injection. These processes went on twice a week. After eight weeks, the liver were perfused with collagenase IV and purified by density gradient centrifugation with Nycodenz for separate HSC. The level of IL-10 was measured by ELISA method; The expression of PDGF and TGFbeta(1) in HSC was detected by semi-quantitative RT-PCR and Western-blot methods.</p><p><b>RESULTS</b>The level of IL-10 in therapy group (adenovirus vector encoding IL-10 gene group) was higher than that in non-therapy group (adenovirus vector without IL-10 gene and PBS group); The expression of TGFbeta(1) mRNA, TGFbeta(1) protein and PDGF mRNA, PDGF protein in therapy group were significantly lower than that in non-therapy group (P < 0.05).</p><p><b>CONCLUSION</b>Downregulating the TGFbeta(1) and PDGF expression could be the passageway by which IL-10 alleviate the degree of proliferation and activation in hepatic stellate cells.</p>
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Physiology / Therapeutics / Platelet-Derived Growth Factor / RNA, Messenger / Transfection / Genetic Therapy / Down-Regulation / Transforming Growth Factor beta Limits: Animals Language: Chinese Journal: Chinese Journal of Surgery Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Physiology / Therapeutics / Platelet-Derived Growth Factor / RNA, Messenger / Transfection / Genetic Therapy / Down-Regulation / Transforming Growth Factor beta Limits: Animals Language: Chinese Journal: Chinese Journal of Surgery Year: 2005 Type: Article