Visual detection of murray valley encephalitis virus by reverse transcription loop-mediated isothermal amplification / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 227-230, 2015.
Article
in English
| WPRIM
| ID: wpr-264595
ABSTRACT
A sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of Murray valley encephalitis virus (MVEV) infection. The reaction was performed in one step in a single tube at 63 °C for 60 min with the addition of the hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was 100 copies per reaction based on 10-fold dilutions of in vitro transcribed RNA derived from a synthetic MVEV DNA template. No cross-reaction was observed with other encephalitis-associated viruses. The assay was further evaluated using spiked cerebrospinal fluid sample with pseudotype virus containing the NS5 gene of MVEV.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription, Genetic
/
Base Sequence
/
Encephalitis Virus, Murray Valley
/
DNA Primers
/
Limit of Detection
/
Genetics
Type of study:
Diagnostic study
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2015
Type:
Article
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