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Visual detection of murray valley encephalitis virus by reverse transcription loop-mediated isothermal amplification / 生物医学与环境科学(英文)
Article in English | WPRIM | ID: wpr-264595
Responsible library: WPRO
ABSTRACT
A sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of Murray valley encephalitis virus (MVEV) infection. The reaction was performed in one step in a single tube at 63 °C for 60 min with the addition of the hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was 100 copies per reaction based on 10-fold dilutions of in vitro transcribed RNA derived from a synthetic MVEV DNA template. No cross-reaction was observed with other encephalitis-associated viruses. The assay was further evaluated using spiked cerebrospinal fluid sample with pseudotype virus containing the NS5 gene of MVEV.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Transcription, Genetic / Base Sequence / Encephalitis Virus, Murray Valley / DNA Primers / Limit of Detection / Genetics Language: English Journal: Biomedical and Environmental Sciences Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Transcription, Genetic / Base Sequence / Encephalitis Virus, Murray Valley / DNA Primers / Limit of Detection / Genetics Language: English Journal: Biomedical and Environmental Sciences Year: 2015 Type: Article