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A PCR-based Rapid Neutralization Assay for GII.4 Norovirus Infection in HIEC6 Cell Culture / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 219-221, 2015.
Article in English | WPRIM | ID: wpr-264597
ABSTRACT
Because of limited viral replication and lack of cytopathic effect in cell culture, a new PCR-based rapid seroneutralization assay for detection of GII.4 norovirus neutralized antibodies was developed with serum samples from acute-phase patients, convalescent-phase patients and healthy controls. According to this study, neutralizing antibodies were detected in 100% of convalescent-phase sera, and in 2.5% of healthy controls sera. However, all of the acute-phase serum samples could not neutralize virus efficiently. Compared to the results from ELISA (96.2% at sensitivity and 80% at specificity), the present in vitro neutralization assay is more specific and more sensitive.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Virology / Enzyme-Linked Immunosorbent Assay / Base Sequence / Case-Control Studies / Cell Line / Polymerase Chain Reaction / Sensitivity and Specificity / DNA Primers / Caliciviridae Infections / Norovirus Type of study: Diagnostic study / Observational study Limits: Humans Language: English Journal: Biomedical and Environmental Sciences Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Virology / Enzyme-Linked Immunosorbent Assay / Base Sequence / Case-Control Studies / Cell Line / Polymerase Chain Reaction / Sensitivity and Specificity / DNA Primers / Caliciviridae Infections / Norovirus Type of study: Diagnostic study / Observational study Limits: Humans Language: English Journal: Biomedical and Environmental Sciences Year: 2015 Type: Article