The feasibility of sputum transportation system in China: effect of sputum storage on the mycobacterial detection / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 982-986, 2014.
Article
in En
| WPRIM
| ID: wpr-264626
Responsible library:
WPRO
ABSTRACT
Sputum transportation from county-level to prefecture-level is an ideal strategy to cover the shortage of the laboratory capability in the resource-poor setting. Here, we firstly evaluated the feasibility of sputum transportation system in China by analyzing the culture and molecular diagnosis results from 1982 smear-positive patients with different delay in processing for culture. In this study, the total contamination rate was 2.32% and the total smear positive/culture negative (S+/C-) rate was 7.57%. We found that sputum specimens refrigerated for no more than 7 d before mycobacterial detection did not affect culture significantly. In addition, the invalid result rates among 0-3 d, 3-7 d, and 7+ d group were 3.63%, 3.14%, and 12.48%, respectively. Statistic analysis revealed that molecular diagnostic results while the invalid result rate of genechip for the specimen with more than 7 d delay was significantly higher (P<0.001). The refrigerators equipped in county laboratories, transport at low temperature and frequent transport services once a week will ensure the feasibility of sputum transportation system in China.
Full text:
1
Index:
WPRIM
Main subject:
Specimen Handling
/
Sputum
/
Transportation
/
China
/
Feasibility Studies
/
Microbiology
/
Mycobacterium
Type of study:
Diagnostic_studies
Limits:
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
Biomedical and Environmental Sciences
Year:
2014
Type:
Article