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Molecular characterization and drug-resistance of Mycobacterium tuberculosis strains in Xuzhou, China / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 960-964, 2014.
Article in English | WPRIM | ID: wpr-264631
ABSTRACT
To understand the genetic diversity and drug resistance status of Mycobacterium tuberculosis (M. tuberculosis) circulating in Xuzhou of China, the spacer-oligonucleotide typing (Spoligotyping) and multi-loci VNTRs (variable number tandem repeats) analysis (MLVA) were utilized for the genotyping of the isolates. Drug susceptibility test (DST) was performed by the proportion method on the Lowenstein-Jensen (L-J) medium using isoniazid, rifampicin, ethambutol, and streptomycin. By Spoligotyping, 287 M. tuberculosis isolates were differentiated into 14 clusters. Then with 15-loci MLVA, these strains could be divided into 32 clusters, 228 genotypes. Of 15 VNTRs, 6 loci had the highly discriminatory powers, 6 loci presented moderate discrimination and 3 loci demonstrated less polymorphism. The DST results showed that 46 strains were resistant to at least one first-line anti-tuberculosis agent. There was a difference in the isoniazid resistance between Beijing and non-Beijing genotype strains. We concluded that the combination of Spoligotyping and 15 VNTR loci as the genotyping in our study was applicable for this region, the drug resistant isolates were identified, and the Beijing family was the most prevalent genotype in the rural counties of Xuzhou.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: China / Drug Resistance, Bacterial / Genotyping Techniques / Genetics / Mycobacterium tuberculosis Type of study: Prognostic study Country/Region as subject: Asia Language: English Journal: Biomedical and Environmental Sciences Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: China / Drug Resistance, Bacterial / Genotyping Techniques / Genetics / Mycobacterium tuberculosis Type of study: Prognostic study Country/Region as subject: Asia Language: English Journal: Biomedical and Environmental Sciences Year: 2014 Type: Article