Neuropeptide Y promotes TGF-beta1 production in RAW264.7 cells by activating PI3K pathway via Y1 receptor / 神经科学通报·英文版

ABSTRACT

<p><b>OBJECTIVE</b>To examine the effect of neuropeptide Y (NPY) on TGF-beta1 production in RAW264.7 macrophages.</p><p><b>METHODS</b>Enzyme linked immunosorbent assay (ELISA) was used to detect TGF-beta1 production. Cell counting kit 8 (CCK-8) was used to assay the viability of RAW264.7 cells. Western blot was used to detect the phosphorylation of PI3K p85.</p><p><b>RESULTS</b>NPY treatment could promote TGF-beta1 production and rapid phosphorylation of PI3K p85 in RAW264.7 cells via Y1 receptor. The elevated TGF-beta1 production induced by NPY could be abolished by wortmannin pretreatment.</p><p><b>CONCLUSION</b>NPY may elicit TGF-beta1 production in RAW264.7 cells via Y1 receptor, and the activated PI3K pathway may account for this effect.</p>