Study on absorption mechanism of genistein self-microemulsifying system in rat intestines / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1406-1409, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-264869
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the absorption mechanism of genistein self-microemulsifying system in rat intestines.</p><p><b>METHOD</b>The concentrations of phenol red and genistein by in situ perfusion in rats were determined by UV and HPLC, respectively. The effects of drug concentrations, pH, various intestinal segments and P-glycoprotein (P-gp) inhibitor verapamil on the absorption had been studied.</p><p><b>RESULT</b>The absorption rate constant (Ka) of genistein had no significant difference at concentrations of 0.05-0.5 mg x mL(-1) and pH of 5.4-7.8 in perfusion. It was Ka of jejunum > ileum > duodenum > colon. The absorption of genistein in jejunum had significant difference (P < 0.05) compared with other parts of intestines. Ka was increased obviously when verapamil was coper-fused with genistein (P < 0. 05).</p><p><b>CONCLUSION</b>The absorption of genistein self-microemulsifying system is a first order process with passive diffusion mechanism related to P-gp efflux. It can be absorbed at all segments of rat intestine, and the jejunum is the best absorption segment, pH had no special effect on the absorption of genistein self-microemulsifying system in rat intestine.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Specificity
/
Pharmacology
/
Temperature
/
Pharmacokinetics
/
Verapamil
/
Chromatography, High Pressure Liquid
/
Rats, Wistar
/
Genistein
/
ATP Binding Cassette Transporter, Subfamily B
/
Emulsions
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2008
Type:
Article
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