Analysis of matrine and oxymatrine in Kushensu injection by UPLC/Q-TOF-MS / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1398-1401, 2008.
Article
in Zh
| WPRIM
| ID: wpr-264871
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for the determination of matrine and oxymatrine in Kushensu injection by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS).</p><p><b>METHOD</b>The analysis was performed on a Acquity UPLC BEH C18 column (2.1 mm x 100 mm, 1.7 microm) with the gradient elution of 10 mmol x L(-1) amine acetate (pH 8, adjusted by aqueous ammonia) and methanol. The flow rate was set as 0.30 mL x min(-1). The column temperature was maintained at 30 degrees C. The peaks were detected at 210 nm and the injection volume was 2 microL.</p><p><b>RESULT</b>The calibration curves showed good linearity within the test ranges of 0.084-3.36 microg for matrine and 0.086-3.44 microg for oxymatrine, respectively. The mean recoveries were 103.2%, 98.7%, and the RSD were 1.5%, 1.2%, respectively.</p><p><b>CONCLUSION</b>The established method is simple, rapid and sensitive, can be used for the quality control of matrine and oxymatrine in Kushensu injection through the manufacturing process and clinical implement.</p>
Full text:
1
Index:
WPRIM
Main subject:
Quinolizines
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Time Factors
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Calibration
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Drugs, Chinese Herbal
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Linear Models
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Chemistry
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Reproducibility of Results
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Chromatography, High Pressure Liquid
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Sensitivity and Specificity
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Spectrometry, Mass, Electrospray Ionization
Type of study:
Diagnostic_studies
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Prognostic_studies
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2008
Type:
Article