Construction and analysis of root cDNA library in Glycyrrhiza uralensis / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To screen and isolate secondary metabolite biosynthesis-related gene for establishing the foundation of functional gene research, we construct a cDNA library of Glycyrrhiza uralensis.</p><p><b>METHOD</b>Total RNA was isolated from G. uralensis using the method of lithium chloride sedimentation. Double strand cDNA was joined into pBlueScript II vector. The number of clones, recombinant rate and length of insert fragments were determined.</p><p><b>RESULT</b>The capacity of the original library was 1.15 x 10(7) with a recombinant rate of 98.2% and the inserted cDNA fragments ranged from 0.5 to 4.8 kb. 126 ESTs through random sequencing were obtained. The most homological proteins came from leguminous plants, including Arabidopsis thaliana, Oryza sativa, and so on. Most of the proteins were related to genes linking cell matabolism, resistance, growth retardation and dormancy.</p><p><b>CONCLUSION</b>The library has enough capacity, high recombinant rate and long insert fragment for the further study.</p>