MIP-1α promotes the migration ability of Jurkat cell through human brain microvascular endothelial cell monolayer / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 35-39, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-264954
ABSTRACT
This study was purposed to explore the mechanism of central nervous system (CNS) leukemia resulting from brain metastasis of human acute T-cell leukemia (T-ALL) cells and the role of MIP-1α in migration of Jurkat cells through human brain microvascular endothelial cells (HBMEC). The real-time PCR, siRNA test, transendothelial migration test, endothelial permeability assay and cell adhesion assay were used to detect MIP-1α expression, penetration and migration ability as well as adhesion capability respectively. The results showed that the MIP-1α expression in Jurkat cells was higher than that in normal T cells and CCRF-HSB2, CCRF-CEM , SUP-T1 cells. The MIP-1α secreted from Jurkat cells enhanced the ability of Jurkat cells to penetrate through HBMEC, the ability of Jurkat cells treated by MIP-1α siRNA to adhere to HBMEC and to migrate trans endothelial cells decreased. It is concluded that the MIP-1α secreted from Jurkat cells participates in process of penetrating the Jurkat cells through HBMEC monolayer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Brain Neoplasms
/
Endothelium, Vascular
/
Cell Adhesion
/
Cell Movement
/
Jurkat Cells
/
Endothelial Cells
/
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
/
Chemokine CCL3
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2014
Type:
Article
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