Long term in-vitro expansion reduces immune modulation function of placental chorionic villi mesenchymal stem cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1552-1556, 2013.
Article
in Zh
| WPRIM
| ID: wpr-264977
Responsible library:
WPRO
ABSTRACT
The main aim of this study was to investigate the biological activities and immune modulation changes of chorionic villi mesenchymal stem cells (CV-MSC) after long term culture. The morphology of the CV-MSC of passage 3 and passage 9 were observed by microscopy, and their phenotypes were detected by flow cytometry. CV-MSC of passage 3 and 9 were co-cultured with PHA-stimulated PBMNC, and IFN-γ concentration in culture medium was detected by ELISA. The mRNA expression of COX-2, HGF and HLA-G in CV-MSC were detected by real-time PCR. The results showed that after long term culture, the CV-MSC kept the MSC morphology and most of the phenotypes including CD31, CD34, CD44, CD45, CD62L, CD73, CD90, CD105, CD117, CD151, CD235a, CD271 and HLA-DR, while the CD49d was significantly up-regulated. Immune modulation ability of CV-MSC was reduced and the mRNA expression of COX-2 and HGF was down regulated after long term culture, but the expression of HLA-G did not found to be obvious change. It is concluded that the long term in vitro expansion changes the expression of CD49d and reduces immune modulation of CV-MSC.
Full text:
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Index:
WPRIM
Main subject:
Placenta
/
Monocytes
/
Cells, Cultured
/
Chorionic Villi
/
Cell Biology
/
Integrin alpha4
/
Allergy and Immunology
/
Mesenchymal Stem Cells
/
Metabolism
Limits:
Female
/
Humans
/
Pregnancy
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2013
Type:
Article