Source and effect of IL-22 in graft versus host disease mouse after allo-genetic bone marrow transplantation / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1526-1529, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-264982
ABSTRACT
This study was purposed to explore the cellular source of IL-22 in graft versus host disease (GVHD) mouse following allo-genetic bone marrow transplantation. BALB/c and C57BL/6 mice were used as recipients and donors, respectively. GVHD model was established by irradiated BABL/c mice inoculated with mixed suspension of C57BL/6 bone marrow cells and splenic lymphocytes. The mice were divided into normal group (normal), total body irradiated group (TBI), bone marrow cell-transplanted group (BMT), and the combination of bone marrow cell and splenic lymphocytes-induced GVHD group (BS). The level of IL-22 in plasma was detected by ELISA. The cellular source of IL-22 and IL-22(+) subsets were detected by flow cytometry. The results showed that compared with normal mice, the level of IL-22 in plasma from BS mice was the highest (P < 0.01). All the lymphocytes of spleen, lymph nodes and peripheral blood from BS mice could produce IL-22, in which the percentage of IL-22(+)CD4(+) T cell was higher than that of IL-22(+)CD8(+) T cells. Not only Th22 cells but also Th1 and Th17 cells were the cellular source of IL-22 in GVHD mice. It is concluded that the high level of IL-22 has been found in mice with GVHD, which mainly originates from IFN-γ(-)IL-17(-)IL-22(+) Th22 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transplantation, Homologous
/
Blood
/
Bone Marrow Transplantation
/
Interleukins
/
Graft vs Host Disease
/
Methods
/
Mice, Inbred BALB C
/
Mice, Inbred C57BL
Limits:
Animals
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2013
Type:
Article
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