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Inhibitory effect of PD98059 on MAPK signaling pathway in acute lymphocytic leukemia cells / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1399-1402, 2013.
Article in Chinese | WPRIM | ID: wpr-265005
ABSTRACT
This study was purposed to investigate the effect of blocking Ras/Erk signaling pathway on expression of important transcription factor c-fos, c-jun and TAK1 gene in primary acute lymphocytic leukemia (ALL) cells. The best effective concentration and effect time of PD98059 were screened; the expression levels of c-fos, c-jun and TAK1 in primary cultured cells of normal persons, primary cultured ALL cells and primary cultured ALL cells treated by PD98059 were detected by SYBR GreenI real-time quantitative-PCR. The results showed that before treatment by PD98059 the expression levels of c-fos and TAK1 mRNA were significantly up-regulated in primary cultured ALL cells as compared with primary cultured cells of normal persons (P = 0.014 and P = 0.017 respectively). After treatment by PD98059, the expression levels of c-fos, c-jun mRNA decreased in all 7 serum samples, while expression of TAK1 was down-regulated in 5 samples, and up-regulated in 2 samples. After treatment with PD98059, there was no statistical difference of c-fos, c-jun and TAK1 expression levels in primary cultured ALL cells and primary cultured normal cells. It is concluded that the c-fos and TAK1 activity of primary cultured ALL cells increases, and blocking the Ras/Erk signaling pathway of ALL cells can lead to obvious decrease of important transcription factors c-fos, c-jun, TAK1 genes expression.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Flavonoids / Tumor Cells, Cultured / Proto-Oncogene Proteins c-jun / Proto-Oncogene Proteins c-fos / MAP Kinase Kinase Kinases / MAP Kinase Signaling System / Precursor Cell Lymphoblastic Leukemia-Lymphoma / Metabolism Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Flavonoids / Tumor Cells, Cultured / Proto-Oncogene Proteins c-jun / Proto-Oncogene Proteins c-fos / MAP Kinase Kinase Kinases / MAP Kinase Signaling System / Precursor Cell Lymphoblastic Leukemia-Lymphoma / Metabolism Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2013 Type: Article