The effect of silicon dioxide on the activation of nuclear factor-kappaB in THP-1 cells / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 179-181, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-265085
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of silicon dioxide (SiO(2)) on the activation of nuclear factor-kappaB (NF-kappaB) in THP-1 cell line.</p><p><b>METHODS</b>THP-1 cells were incubated with a series of doses of SiO(2) (0, 100, 200 micro g/ml). The location of NF-kappaB p65 subunit (NF-kappaB/p65) in THP-1 cells was detected by immunofluorescence and laser scanning confocal microscope (LSCM). The expression of NF-kappaB/p65 in nuclei was measured by Western blot analysis.</p><p><b>RESULTS</b>The majority of fluorescein isothiocyanate (FITC)-labelled NF-kappaB/p65 located in the nuclei 30 min after stimulation by 100 micro g/ml SiO(2), whereas the FITC-labelled NF-kappaB/p65 were mainly seen in the plasma of normal control cells. The expression of NF-kappaB/p65 in THP-1 nuclear protein was low in control group (0 micro g/ml SiO(2)) while it increased after stimulation by 100 micro g/ml SiO(2) and 200 micro g/ml SiO(2) for 15 min and 30 min. The level of NF-kappaB/p65 was comparatively increased with the increasing of doses and time. Lipopolysaccharides (LPS), an activator of NF-kappaB, had similar effect as SiO(2) on the activation of NF-kappaB/p65 in THP-1 cells.</p><p><b>CONCLUSION</b>SiO(2) could activate and internalize NF-kappaB in the THP-1 cell line.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Cell Line
/
Cell Nucleus
/
Blotting, Western
/
NF-kappa B
/
Silicon Dioxide
/
Microscopy, Confocal
/
Fluorescent Antibody Technique, Indirect
/
Dose-Response Relationship, Drug
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS