Preliminary study on human embryonic kidney cell line HEK-293 after porcine endogenous retrovirus infection / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 220-223, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-265145
ABSTRACT
<p><b>OBJECTIVE</b>To assess the infectivity of porcine endogenous retrovirus (PERV) via in vitro infection of human embryonic kidney cell line HEK-293.</p><p><b>METHODS</b>PERV particles were detected by immunoelectron microscopy. PERV DNA and mRNA were studied in HEK-293 24 hours after the infection using polymerase chain reaction and reverse transcriptase-PCR respectively. The PERV types were also analyzed. PERV-gag protein was observed by confocal microscopy.</p><p><b>RESULTS</b>Retroviral particles were round under electron microscope. PERV-gag pol gene and gag protein were detected and expressed in the infected HEK-293 cells. The types of PERV were PERV-A and PERV-B. PERV-gag protein was also identified in the cytoplasm of infected cells by confocal microscopy.</p><p><b>CONCLUSIONS</b>PERV is able to infect HEK-293 cell line in vitro; types of PERV-gag protein is also expressed as a result. Further studies are thus necessary in order to evaluate the possibility of xenozoonoses in pig-to-human xenotransplantation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Swine
/
Virology
/
Virulence
/
DNA, Viral
/
RNA, Messenger
/
Gene Products, gag
/
Genes, gag
/
Cell Line
/
Gene Amplification
/
Endogenous Retroviruses
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2005
Type:
Article
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