Aminoglycoside ototoxicity in three murine strains and effects on NKCC1 of stria vascularis / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 980-985, 2006.
Article
in English
| WPRIM
| ID: wpr-265267
ABSTRACT
<p><b>BACKGROUND</b>After establishing a murine model of aminoglycoside antibiotic (AmAn) induced ototoxicity, the sensitivity of AmAn induced ototoxicity in three murine strains and the effect of kanamycin on the expression of Na-K-2Cl cotransporter-1 (NKCC1) in stria vascularis were investigated.</p><p><b>METHODS</b>C57BL/6J, CBA/CaJ, NKCC1(+/-) mice (24 of each strain) were randomly divided into four experimental groups A kanamycin alone; B kanamycin plus 2, 3-dihydroxybenzoate; C 2, 3-dihydroxybenzoate alone; and D control group. Mice were injected with kanamycin or/and 2, 3-dihydroxybenzoate twice daily for 14 days. Auditory brainstem response (ABR) was measured and morphology of cochlea delineated with succinate dehydrogenase staining. Expression of NKCC1 in stria vascularis was detected immunohistochemically.</p><p><b>RESULTS</b>All three strains in groups A and B developed significant ABR threshold shifts (P < 0.01), which were accompanied by outer hair cell loss. NKCC1 expression in stria vascularis was the weakest in group A (A cf D, P < 0.01) and the strongest in groups C and D (P < 0.05). CBA/CaJ mice had the highest sensitivity to AmAn.</p><p><b>CONCLUSIONS</b>Administration of kanamycin established AmAn induced ototoxicity. Kanamycin inhibited the expression of NKCC1 in stria vascularis. 2, 3-dihydroxybenzoate attenuated AmAn induced ototoxicity-possibly by enhancing the expression of NKCC1. Age related hearing loss did not show additional sensitivity to AmAn induced ototoxicity in murine model.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Auditory Threshold
/
Stria Vascularis
/
Kanamycin
/
Chemistry
/
Hair Cells, Vestibular
/
Sodium-Potassium-Chloride Symporters
/
Toxicity
/
Solute Carrier Family 12, Member 2
/
Mice, Inbred CBA
/
Mice, Inbred C57BL
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS