Determination of carnosic acid in rat stomach and intestine by high performance liquid chromatography method / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 766-769, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-265309
ABSTRACT
<p><b>OBJECTIVE</b>To establish a HPLC method to determine the carnosic acid in the stomach and intestine of rats and study its tissue distribution characteristics.</p><p><b>METHOD</b>After intragastric administration of carnosic acid (90 mg x kg(-1)), rats for each time-point were sacrificed by decapitation. After removal of the blood, various tissues were rapidly removed and weighted, all tissues were treated with a series of pretreatment before HPLC. Chromatographic separation was achieved on a Kromasil C18 column (4.6 mm x 150 mm, 5 microm) protected by an ODS guard column at 25 degrees C, using acetonitrile-0.1% phosphoric acid solution (5545) as mobile phase, at a flow rate of 1 mL x min(-1). The wavelength of the UV detector was set at 210 nm for carnosic acid and internal standard.</p><p><b>RESULT</b>Good linearities were obtained in every tissue over a range of 0.3212-160.6 mg x L(-1). The recovery, intra-day and inter-day precision and accuracy of three concentrations of carnosic acid in tissues met the requirements of methodology. And the stability of the tissue samples were also validated. The results of distribution in stomach and intestine showed that the highest concentration was (307.1 +/- 119.2) microg x g(-1) in stomach and (33.32 +/- 17.70) microg x g(-1) in intestine after intragastric administration of carnosic acid.</p><p><b>CONCLUSION</b>The HPLC method was established to determine the concentration of carnosic acid in tissues. This method is quick, precise, and reproducible. It is the first time to study the tissue distribution of carnosic acid in rats after intragastric administration.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stomach
/
Time Factors
/
Calibration
/
Pharmacokinetics
/
Plant Extracts
/
Tissue Distribution
/
Linear Models
/
Chromatography, High Pressure Liquid
/
Sensitivity and Specificity
/
Abietanes
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2009
Type:
Article
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